He is also known for his supporting work in comedy films, such as You, Me and Dupree (2006), Hot Rod (2007), Superbad (2007), Forgetting Sarah Marshall (2008), Tropic Thunder (2008), Night at the Museum: Battle of the Smithsonian (2009), Paul (2011), Men in Black 3 (2012), and Maggie's Plan (2015).
He also had a small cameo as the voice of the USS Vengeance computer in 2013's Star Trek Into Darkness.
Hader starred in a dramatic role in the 2014 film The Skeleton Twins, opposite Kristen Wiig, with whom he worked on Saturday Night Live.
He voiced a gazelle in Ice Age: Dawn of the Dinosaurs.
Hader appeared in Night at the Museum: Battle of the Smithsonian as Major General George Armstrong Custer.
He made a small appearance in the 2009 film Year One with Jack Black and Michael Cera.